What color does the reporter protein produced by the plasmid fluoresce?

What color does the reporter protein produced by the plasmid fluoresce?

• A. Green
• B. Blue
• C. Red
• D. Yellow

Answer: (C)

The color of the fluorescence comes from which fluorescent protein is encoded by the reporter gene on the plasmid. If the plasmid carries a red fluorescent protein gene (such as DsRed or mCherry), the expressed reporter protein emits red light when illuminated with the appropriate excitation. This is why the correct color is red—the plasmid is producing a red fluorescent protein. If the plasmid carried a green fluorescent protein gene, you would see green fluorescence instead, and so on for other colors. Red emission from common red fluorescent proteins is typically around wavelengths near 610 nm when excited by blue or green light.

The color of the fluorescence comes from which fluorescent protein is encoded by the reporter gene on the plasmid. If the plasmid carries a red fluorescent protein gene (such as DsRed or mCherry), the expressed reporter protein emits red light when illuminated with the appropriate excitation. This is why the correct color is red—the plasmid is producing a red fluorescent protein. If the plasmid carried a green fluorescent protein gene, you would see green fluorescence instead, and so on for other colors. Red emission from common red fluorescent proteins is typically around wavelengths near 610 nm when excited by blue or green light.