What are the sticky ends generated by BamHI and HindIII?

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Multiple Choice

What are the sticky ends generated by BamHI and HindIII?

Explanation:
Sticky ends come from staggered cuts by restriction enzymes, leaving short single-stranded overhangs that can base-pair with complementary sequences. BamHI recognizes GGATCC and cuts between the first two Gs (G/GATCC), producing a 4-base 5' overhang of GATC. HindIII recognizes AAGCTT and cuts between the two As (A/A GCTT), producing a 4-base 5' overhang of AGCT. So BamHI yields GATC as its overhang and HindIII yields AGCT.

Sticky ends come from staggered cuts by restriction enzymes, leaving short single-stranded overhangs that can base-pair with complementary sequences. BamHI recognizes GGATCC and cuts between the first two Gs (G/GATCC), producing a 4-base 5' overhang of GATC. HindIII recognizes AAGCTT and cuts between the two As (A/A GCTT), producing a 4-base 5' overhang of AGCT. So BamHI yields GATC as its overhang and HindIII yields AGCT.

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