How do you calculate the dilution factor for a two-step dilution with 1:10 and 1:100 steps?

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Multiple Choice

How do you calculate the dilution factor for a two-step dilution with 1:10 and 1:100 steps?

Explanation:
Serial dilutions multiply. A 1:10 step reduces concentration by a factor of 10, and a 1:100 step reduces by a factor of 100. Doing them in sequence, you multiply the factors: 10 × 100 = 1000. So the overall dilution is 1000-fold, meaning the final concentration is one thousandth of the original. The other numbers reflect either only one step (100-fold) or express the remaining fraction rather than the dilution factor (1/1000 is the final fraction, not the dilution factor).

Serial dilutions multiply. A 1:10 step reduces concentration by a factor of 10, and a 1:100 step reduces by a factor of 100. Doing them in sequence, you multiply the factors: 10 × 100 = 1000. So the overall dilution is 1000-fold, meaning the final concentration is one thousandth of the original. The other numbers reflect either only one step (100-fold) or express the remaining fraction rather than the dilution factor (1/1000 is the final fraction, not the dilution factor).

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