How do the R+ and R- tubes differ in a restriction digest setup?

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Multiple Choice

How do the R+ and R- tubes differ in a restriction digest setup?

Explanation:
Restriction enzymes cut DNA at specific sites, producing smaller DNA fragments. In the digest setup, the tube with the enzyme (R+) undergoes digestion and yields smaller fragments, while the tube without the enzyme (R-) remains undigested and shows larger DNA pieces. So the key difference is that enzyme presence reduces fragment size in the R+ sample compared to the R- sample.

Restriction enzymes cut DNA at specific sites, producing smaller DNA fragments. In the digest setup, the tube with the enzyme (R+) undergoes digestion and yields smaller fragments, while the tube without the enzyme (R-) remains undigested and shows larger DNA pieces. So the key difference is that enzyme presence reduces fragment size in the R+ sample compared to the R- sample.

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